Development of a DIPG Orthotopic Model in Mice Using an Implantable Guide-Screw System.
In this work we set to develop and to validate a new in vivo frameless orthotopic Diffuse Intrinsic Pontine Glioma (DIPG) model based in the implantation of a guide-screw system.It consisted of a guide-screw also called bolt, a Hamilton syringe with a 26-gauge needle and an insulin-like 15-gauge needle.The guide screw is 2.6 mm in length and harbor